Confocal Microscopy on Fluorescent Dipoles
In classical light microscopy, the resolution is limited according to
Abbé by half the wavelength of the light. In the beginning of the last
century, a theory has been developed which predicts an increase of the
resolution by a factor of ten by making use of sophisticated techniques.
In 1982 the idea has been reactivated and first microscopes have been realized.
Through further development it became possible to investigate optical properties
of single molecules, what may become important for medicine and molecular
biology. In the present laboratory the imaging of single molecules due to this
new technique is simulated.
This module has been developed within the framework of a diploma thesis
at the University of Basel. Especially interesting are the
Pattern Generator and the animated sketch of the
relation between Spherical and Carthesian Coordinates
Koordinaten in three dimensions.