Confocal Microscopy on Fluorescent Dipoles

In classical light microscopy, the resolution is limited according to Abbé by half the wavelength of the light. In the beginning of the last century, a theory has been developed which predicts an increase of the resolution by a factor of ten by making use of sophisticated techniques. In 1982 the idea has been reactivated and first microscopes have been realized. Through further development it became possible to investigate optical properties of single molecules, what may become important for medicine and molecular biology. In the present laboratory the imaging of single molecules due to this new technique is simulated.

This module has been developed within the framework of a diploma thesis at the University of Basel. Especially interesting are the Pattern Generator and the animated sketch of the relation between Spherical and Carthesian Coordinates Koordinaten in three dimensions.